Effect of Combination of Energy Levels in Feed and Duck Egg Yolk as a Semen Diluent on the Quality of Native Rooster Spermatozoa
Keywords:
semen diluent, egg yolk, spermatozoa quality, Metabolic energyAbstract
This study aims to determine the effect of energy levels in feed and the use of duck egg yolks as a semen diluent on the quality of spermatozoa. The material used is 80 weeks old native rooster, eosin, 3% NaCl, duck egg yolk and aquadest. This research is a biological research using a 2x4 factorial completely randomized design (CRD). The first factor was the energy level in the feed (E) with 2 levels (2750 and 2850kcal/kg) and the second factor was the level of egg yolk as a semen diluent (P) (0, 4 and 8 ml/ml semen). Semen was collected using a massage method from the abdomen to the cloaca. The results of statistical analysis showed that the energy level in the feed had a very significant effect (p<0.01) on the movement of spermatozoa and significantly (p<0.05) on live spermatozoa. Dilution using egg yolk from duck eggs gave a very significant effect (p<0.01) on the pH of semen and the number of live spermatozoa. The average pH of the semen obtained in this study ranged from 6.00 – 8.83; mass movement score 2.25-3.00; spermatozoa motility by 80%; live spermatozoa were 89.50-92.50% and spermatozoa abnormalities were 2.27-2.28%. This study concluded that the quality of semen can be improved by increasing the energy level in the feed and duck egg yolk can be used as a semen diluent. The best energy level to improve semen quality is 2850 kcal/kg and egg yolk as a semen diluent can be used up to 4ml/ml semen.
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